Not to be confused with Transcription Translation is the synthesis of protein from mRNA template obtained from the transcription process. The translation process is carried out in an amino acid polymerizing organelle called ribosome. Translation marks the completion of the process of gene expression. [toc] Proteins perform various activities in the body and are classified based on their functions in living cells into antibodies that fight infections within our body, enzymes that speed up chemical reactions within the cells, structural proteins such as collagen, keratin and elastin that provide structural support for the bone, skin and tendons respectively, contractile proteins such as actin and myosin found in the muscles, hormones that regulates the activities of the body organs, storage proteins such as ferritin which stores iron and ovalbumin that help store amino acids and transport proteins that help transports biochemical molecules from cell to cell.
What is RNA?RNA (Ribonucleic acid) is a single-stranded nucleic acid containing ribose sugar. See the nucleic acid. There are three types of RNAs: tRNA, mRNA and rRNA.
Difference between RNA and DNAThe distinct characteristics of RNAs that differentiates them from DNA (Deoxyribonucleic acid) are the possession of ribose sugar and nucleotide base uracil (U) in place of thymine (T)
Difference between tRNA, mRNA and rRNAmRNAs are made from DNA template during transcription, which later serves as a template for protein synthesis. tRNAs perform a vital role in translation, it carries anti-codons that are complementary to the codon on the mRNA during protein synthesis. rRNAs are abundant in the ribosome, they together with protein make up the cell organelle called ribosome. However, another major difference between these RNAs is their post-transcriptional modification. For example, mRNAs are methylated at the 5’ end and modified at 3’ end with multiple Adenine ( 5’- methylated cap and poly-A tail at 3’ end). While tRNAs have CCA 3’ end that may be modified by methylation.
What are codons?Codons are a set of three adjacent nucleotide bases found on the mRNA that code for a specific amino acid. It is to be the noted that there are 64 known possible codons in mRNA. 61 of which codes for amino acids. Codon AUG or GUG which codes for Methionine and Valine respectively are the start codons in living thing while UAG, UAA and UGA are the stop codons. Important key molecules to consider during translation include: messenger RNA (mRNA), ribosome, tRNA, release factors The mRNA, the substrate for translation have a unique 3’ ends called the Poly-A tail. mRNA also contains codons that codes for specific amino acids. At the 5’ end is a methylated cap.
The process is broken into three stages:
- Initiation stage
- Elongation stage
- Termination stage